Large deletions in the CBF gene cluster at the Fr-B2 locus are associated with reduced frost tolerance in wheat

Pearce, Stephen and Zhu, Jie and Boldizsár, Ákos and Vágújfalvi, Attila and Burke, Adrienne and Galiba, Gábor and Garland‑Campbell, Kimberley and Dubcovsky, Jorge (2013) Large deletions in the CBF gene cluster at the Fr-B2 locus are associated with reduced frost tolerance in wheat. THEORETICAL AND APPLIED GENETICS, 126 (11). pp. 2683-2697. ISSN 0040-5752

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Wheat plants which are exposed to periods of low temperatures (cold acclimation) exhibit increased survival rates when they are subsequently exposed to freezing temperatures. This process is associated with large-scale changes in the transcriptome which are modulated by a set of tandemly duplicated C-repeat Binding Factor (CBF) transcription factors located at the Frost Resistance-2 (Fr-2) locus. While Arabidopsis has three tandemly duplicated CBF genes, the CBF family in wheat has undergone an expansion and at least 15 CBF genes have been identified, 11 of which are present at the Fr-2 loci on homeologous group 5 chromosomes. We report here the discovery of three large deletions which eliminate 6, 9, and all 11 CBF genes from the Fr-B2 locus in tetraploid and hexaploid wheat. In wild emmer wheat, the Fr-B2 deletions were found only among the accessions from the southern sub-populations. Among cultivated wheats, the Fr-B2 deletions were more common among varieties with a spring growth habit than among those with a winter growth habit. Replicated freezing tolerance experiments showed that both the deletion of nine CBF genes in tetraploid wheat and the complete Fr-B2 deletion in hexaploid wheat were associated with significant reductions in survival after exposure to freezing temperatures. Our results suggest that selection for the wild-type Fr-B2 allele may be beneficial for breeders selecting for varieties with improved frost tolerance. © 2013 Springer-Verlag Berlin Heidelberg.

Item Type: Article
Subjects: Q Science / természettudomány > QK Botany / növénytan
Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan
Depositing User: MTMT SWORD
Date Deposited: 05 Dec 2013 14:28
Last Modified: 09 Dec 2013 12:15

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