Molecular genetic study on a historical Solanum (Solanaceae) herbarium specimen collected by Paulus Kitaibel in the 18th century

Poczai, P. and Taller, J. and Szabó, I. (2009) Molecular genetic study on a historical Solanum (Solanaceae) herbarium specimen collected by Paulus Kitaibel in the 18th century. Acta Botanica Hungarica, 51 (3-4). pp. 337-346. ISSN 0236-6495

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Abstract

The herbarium of Paulus Kitaibel (1757–1817) has been preserved in good condition and can still be used for the investigation of problematic questions. There happens to be a misidentified specimen on the back of one of the sheets. Although it has been studied by several botanists, it has proved impossible to identify this species because important morphological traits have been lost due to the age of the preserved material. The complete ITS region of the over 200-year-old herbarium specimen has been amplified by PCR. Sequence analysis and the secondary structure modelling of the ITS2 RNA transcript clearly provided, that the specimen is Solanum scabrum , thus settling the controversy surrounding this question. This result is particularly interesting, as the presence of S. scabrum in the Hungarian flora, has never been officially documented.

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