Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR

Erdősi, Orsolya and Szakmár, Katalin and Szili, Zsuzsanna and Szita, Géza and Bernáth, Sándor and Sövényi, József and Laczay, Péter (2018) Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR. Acta Veterinaria Hungarica, 66 (1). pp. 1-11. ISSN 0236-6290

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Abstract

The rapid detection of Campylobacter spp. is of utmost importance for the reduction of infections in humans by contaminated food products. The standard culturing method (ISO 10272-1:2006) involves a high time and labour demand. In this paper, we present a method that reduces the detection time of Campylobacter spp. to or below one third as compared to the ISO method, at a reduced cost per test. We used redox potential change of enrichment cultures (Bolton broth with Bolton selective supplement) for reliably selecting Campylobacter-contaminated raw milk and broiler meat samples. Identification of Campylobacter spp. in the contaminated samples was done by real-time PCR method. Culturing time to conclusive redox monitoring varied between 6 and 24 h for positive samples, depending on the contamination rate, in contrast to 136 h with the standard culturing process. However, now the Campylobacter-negative majority of food samples will not need to be tested by real-time PCR because redox potential monitoring can identify them in the selective enrichment phase. This method could be potentially used as a faster alternative to the current standard ISO 10272-1:2006, for nonregulatory monitoring purposes.

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