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Caffeine strongly improves motility parameters of turkey spermatozoa with no effect on cell viability

Slanina, Tomáš and Miškeje, Michal and Tirpák, Filip and Błaszczyk, Martyna and Formicki, Grzegorz and Massányi, Péter (2018) Caffeine strongly improves motility parameters of turkey spermatozoa with no effect on cell viability. Acta Veterinaria Hungarica, 66 (1). pp. 137-150. ISSN 0236-6290

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Abstract

The purpose of this study was to evaluate the impact of caffeine on turkey spermatozoa during in vitro incubation. Experimental samples were prepared by diluting the raw semen with nine different concentrations of caffeine – from 0.078125 mg/mL to 10 mg/mL. The individual motility parameters were evaluated by the Computer Assisted Semen Analyser (CASA) system, and the viability of spermatozoa was evaluated using eosin-nigrosin staining. Selected parameters were recorded at six time periods: 0, 1, 2, 3, 4 and 5 h at 5 °C and 41 °C. A significantly higher motility and progressive motility of spermatozoa (P < 0.01 and P < 0.001, respectively) was detected in the samples containing caffeine ranging from 0.15625 to 7.5 mg/mL as compared to the control sample at 5 °C. At an incubation temperature of 41 °C the positive effect of caffeine on motility parameters was observed only at the beginning of incubation (at times 0 and 1). The tested caffeine concentrations showed no significant effect on the viability of turkey spermatozoa at any time period of incubation. A higher percentage of dead spermatozoa was observed for incubation at 41 °C (from 5.96% to 11.1%) in comparison to 5 °C (from 1.62% to 5.79%). The results suggest that caffeine can be used as a suitable component of turkey semen extenders and has the potential to improve fertility.

Item Type: Article
Additional Information: MTA KFB támogatási szerződés alapján archiválva
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: Violetta Baliga
Date Deposited: 10 Apr 2018 11:35
Last Modified: 31 Mar 2019 00:16
URI: http://real.mtak.hu/id/eprint/79132

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