Utilization of agro-industrial residues for the production of β-Galactosidase using fungal isolate under solid state fermentation conditions

Kaur, R. and Panesar, P.S. and Singh, R.S. (2018) Utilization of agro-industrial residues for the production of β-Galactosidase using fungal isolate under solid state fermentation conditions. Acta Alimentaria, 47 (2). pp. 162-170. ISSN 0139-3006

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Abstract

β-Galactosidase is an enzyme of commercial importance owing to its multiple benefits. Among all microbial sources, fungal species are of great interest for the production of this enzyme. Thus, the aim of this present work was to optimize the media as well as process parameters to achieve maximum β-Galactosidase production by solid state fermentation using the fungal isolate Rhizomucor pusillus. Various agro-industrial residues were tested for carbon as well as for nitrogen sources. The different process parameters were also studied to observe their effects on β-galactosidase production. Among all screened agro-industrial residues, wheat bran and corn steep liquor had the potential to be used as carbon and nitrogen sources, respectively; whereas MgSO₄ was found to be a suitable salt supplement. The optimal process parameters included particle size of 1000 microns, 50% moisture content, pH 5.5, 50 ºC temperature, and 7 days of fermentation.

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