Identification and antimicrobial susceptibility testing of anaerobic bacteria: Rubik's cube of clinical microbiology?

Gajdács, Márió and Spengler, Gabriella and Zsoldiné Urbán, Edit (2017) Identification and antimicrobial susceptibility testing of anaerobic bacteria: Rubik's cube of clinical microbiology? ANTIBIOTICS, 6 (4). p. 25. ISSN 2079-6382


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Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically. In the past, antimicrobial susceptibility of these microorganisms showed predictable patterns and empirical therapy could be safely administered but recently a steady and clear increase in the resistance for several important drugs (beta-lactams, clindamycin) has been observed worldwide. For this reason, antimicrobial susceptibility testing of anaerobic isolates for surveillance purposes or otherwise is of paramount importance but the availability of these testing methods is usually limited. In this present review, our aim was to give an overview of the methods currently available for the identification (using phenotypic characteristics, biochemical testing, gas-liquid chromatography, MALDI-TOF MS and WGS) and antimicrobial susceptibility testing (agar dilution, broth microdilution, disk diffusion, gradient tests, automated systems, phenotypic and molecular resistance detection techniques) of anaerobes, when should these methods be used and what are the recent developments in resistance patterns of anaerobic bacteria.

Item Type: Article
Additional Information: Acknowledgments: This study was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4. A/2-11-1-2012-0001 ‘National Excellence Program.’ G.S. was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. M.G. was supported by the UNKP-17-3 New National Excellence Program of the Ministry of Human Capacities. M.G. has received input for the study/project through ESCMID’s mentorship program by E.U. The authors would like to thank the “Top 35 of Antibiotics Travel Awards 2017” for the opportunity to publish in the Journal.
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: MTMT SWORD
Date Deposited: 14 Sep 2018 06:56
Last Modified: 14 Sep 2018 06:56

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