Label-free Multiscale Transport Imaging of the Living Cell

Osváth, Szabolcs and Herényi, Levente and Agócs, Gergely and Kis-Petik, Katalin and Kellermayer, Miklós (2018) Label-free Multiscale Transport Imaging of the Living Cell. BIOPHYSICAL JOURNAL, 1. S0006-3495(18)30921. ISSN 0006-3495

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The living cell is characterized by a myriad of parallel intracellular transport processes. Simultaneously capturing their global features across multiple temporal and spatial scales is a nearly unsurmountable task. Here we present a method that enables the microscopic imaging of the entire spectrum of intracellular transport on a broad time scale without the need for prior labeling. We show that from the time-dependent fluctuation of pixel intensity, in either bright-field or phase-contrast microscopic images, a scaling factor can be derived that reflects the local Hurst coefficient (H), the value of which reveals the microscopic mechanisms of intracellular motion. The Hurst coefficient image of the interphase cell displays an unexpected, overwhelming superdiffusion (H > 0.5) in the cytoplasm and subdiffusion (H < 0.5) in the nucleus, and provides unprecedented sensitivity in detecting transport processes associated with the living state.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
Depositing User: MTMT SWORD
Date Deposited: 14 Sep 2018 11:41
Last Modified: 14 Sep 2018 11:41

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