Multiple divergent picobirnaviruses with functional prokaryotic Shine- Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken

Boros, Ákos and Polgár, Beáta and Pankovics, Péter and Fenyvesi, Hajnalka and Engelmann, Péter and Phan, Tung Gia and Delwart, Eric and Reuter, Gábor (2018) Multiple divergent picobirnaviruses with functional prokaryotic Shine- Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken. Virology, 525. pp. 62-72. ISSN 0042-6822

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Abstract

Picobirnaviruses (PBVs) of family Picobirnaviridae have bisegmented (S1 and S2 segments), doublestranded RNA genomes. In this study a total of N = 12 complete chicken PBVs (ChPBV) segments (N = 5 of S1 and N = 7 of S2, Acc. Nos.: MH425579-90) were determined using viral metagenomic and RTPCR techniques from a single cloacal sample of a diarrheic chicken. The identified ChPBV segments are unrelated to each other and distant from all of the currently known PBVs. In silico sequence analyses revealed the presence of conserved prokaryotic Shine-Dalgarno-like (SD-like) sequences upstream of the three presumed open reading frames (ORFs) of the S1 and a single presumed ORF of the S2 segments. According to the results of expression analyses in E. coli using 6xHis-tagged recombinant ChPBV segment 1 construct and Western blot these SD-like sequences are functional in vivo suggesting that S1 of study PBVs can contain three ORFs and supporting the bacteriophage-nature of PBVs.

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