REAL

The nonstop decay and the RNA silencing systems operate cooperatively in plants

Szádeczky-Kardoss, István and Csorba, Tibor and Auber, Andor and Schamberger, Anita and Nyikó, Tünde and Taller, János and Orbán, Tamás I. and Burgyán, József and Silhavy, Dániel (2018) The nonstop decay and the RNA silencing systems operate cooperatively in plants. NUCLEIC ACIDS RESEARCH, 46 (9). pp. 4632-4648. ISSN 0305-1048

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Abstract

Translation-dependent mRNA quality control systems protect the protein homeostasis of eukaryotic cells by eliminating aberrant transcripts and stimulating the decay of their protein products. Although these systems are intensively studied in animals, little is known about the translation-dependent quality control systems in plants. Here, we characterize the mechanism of nonstop decay (NSD) system in Nicotiana benthamiana model plant. We show that plant NSD efficiently degrades nonstop mRNAs, which can be generated by premature polyadenylation, and stop codon-less transcripts, which are produced by endonucleolytic cleavage. We demonstrate that in plants, like in animals, Pelota, Hbs1 and SKI2 proteins are required for NSD, supporting that NSD is an ancient and conserved eukaryotic quality control system. Relevantly, we found that NSD and RNA silencing systems cooperate in plants. Plant silencing predominantly represses target mRNAs through endonucleolytic cleavage in the coding region. Here we show that NSD is required for the elimination of 5' cleavage product of mi- or siRNA-guided silencing complex when the cleavage occurs in the coding region. We also show that NSD and nonsense-mediated decay (NMD) quality control systems operate independently in plants.

Item Type: Article
Subjects: Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 29 Sep 2018 06:54
Last Modified: 24 Jan 2019 13:17
URI: http://real.mtak.hu/id/eprint/85920

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