REAL

Characterization of a novel 4.0-kb y-type HMW-GS from Eremopyrum distans

Dai, S.F. and Xu, D.Y. and Wen, Z.J. and Song, Z.P. and Chen, H.X. and Li, H.Y and Li, J.R. and Kang, L.Z. and Yan, Z.H. (2018) Characterization of a novel 4.0-kb y-type HMW-GS from Eremopyrum distans. Cereal Research Communications, 46 (3). pp. 499-509. ISSN 0133-3720

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Abstract

A novel 4.0-kb Fy was sequenced and bacterially expressed. This gene, the largest y-type HMW-GS currently reported, is 4,032-bp long and encodes a mature protein with 1,321 amino acid (AA) residues. The 4.0-kb Fy shows novel modifications in all domains. In the N-terminal, it contains only 67 AA residues, as three short peptides are absent. In the repetitive domain, the undecapeptide RYYPSVTSPQQ is completely lost and the dodecapeptide GSYYPGQTSPQQ is partially absent. A novel motif unit, PGQQ, is present in addition to the two standard motif units PGQGQQ and GYYPTSPQQ. Besides, an extra cysteine residue also occurs in the middle of this domain. The large molecular mass of the 4.0-kb Fy is mainly due to the presence of an extra-long repetitive domain with 1,279 AA residues. The novel 4.0-kb Fy gene is of interest in HMW-GS gene evolution as well as to wheat quality improvement with regard to its longest repetitive domain length and extra cysteines residues.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: Ágnes Sallai
Date Deposited: 02 Oct 2018 05:25
Last Modified: 30 Sep 2019 23:16
URI: http://real.mtak.hu/id/eprint/86437

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