REAL

MODIFIED DOUBLE-DISC SYNERGY TEST (MDDST) FOR DETECTION OF EXTENDED-SPECTRUM BETA-LACTAMASES IN AmpC BETA-LACTAMASE-PRODUCING KLEBSIELLA CLINICAL ISOLATES

Diab, Atef M. and Abul-Aziz, M. H. and El-Kholy, Iman M. A. and Rezk, Mona A. (2018) MODIFIED DOUBLE-DISC SYNERGY TEST (MDDST) FOR DETECTION OF EXTENDED-SPECTRUM BETA-LACTAMASES IN AmpC BETA-LACTAMASE-PRODUCING KLEBSIELLA CLINICAL ISOLATES. EUROPEAN CHEMICAL BULLETIN, 7 (2). pp. 89-92. ISSN 2063-5346

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Abstract

The detection of ESBL(S) in gram-negative bacteria that produce AmpC beta-lactamases is problematic. In the present study, the performance of MDDST that employs a combination of cefepime and piperacillin-tazobactam for the detection of ESBL(S) Klebsiella producing AmpC beta-lactamases was evaluated and compared with DDST. E-test phenotypic confirmatory and MTDT tests were adopted for more data confidence. A total of 100 clinical isolates of Klebsiella, which met the CLSI (2012) screening criteria as having broth microdilution (BMD) MIC of > or =2 mg/mL for at least one extended spectrum cephalosporin [ceftazidime (CAZ), cefotaxime (CTX) and cefpodoxime], were accurately-selected for the study. MDDST detected ESBLs in 62 out of the100 studied isolates with 100% sensitivity and specificity, whereas DDST detected ESBLs in only 52 isolates with 92.9% sensitivity and 100% specificity. E-test could detect ESBLs in 62 isolates, while as many as 34/62 ESBL positive isolates were confirmed to be AmpC beta-lactamase positive by the MTDT. MDDST and E-Test could detect ESBLs in all the 34 AmpC positive isolates, whereas DDST detect ESBLs in only 26 isolates.The study recommended MDDST as superior to DDST for the detection of ESBLs in AmpC beta-lactamase-producing Klebsiella spp. and this was confirmed by MTDT and E-Tests.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 22 Dec 2018 11:14
Last Modified: 22 Dec 2018 11:14
URI: http://real.mtak.hu/id/eprint/88839

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