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Interleukin-6 released from differentiating human beige adipocytes improves browning

Kristóf, Endre and Klusóczki, Ágnes and Veress, Roland and Shaw, Abhirup and Combi, Zsolt Sándor and Varga, Klára and Győry, Ferenc and Balajthy, Zoltán and Bai, Péter and Bacsó, Zsolt and Fésüs, László (2019) Interleukin-6 released from differentiating human beige adipocytes improves browning. EXPERIMENTAL CELL RESEARCH, 377 (1-2). pp. 47-55. ISSN 0014-4827

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Abstract

Brown and beige adipocytes contribute significantly to the regulation of whole body energy expenditure and systemic metabolic homeostasis not exclusively by thermogenesis through mitochondrial uncoupling. Several studies have provided evidence in rodents that brown and beige adipocytes produce a set of adipokines ("batokines") which regulate local tissue homeostasis and have beneficial effects on physiological functions of the entire body. We observed elevated secretion of Interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1, but not tumor necrosis factor alpha (TNFα) or IL-1β pro-inflammatory cytokines, by ex vivo differentiating human beige adipocytes (induced by either PPARγ agonist or irisin) compared to white. Higher levels of IL-6, IL-8 and MCP-1 were released from human deep neck adipose tissue biopsies (enriched in browning cells) than from subcutaneous ones. IL-6 was produced in a sustained manner and mostly by the adipocytes and not by the undifferentiated progenitors. Continuous blocking of IL-6 receptor by specific antibody during beige differentiation resulted in downregulation of brown marker genes and increased morphological changes that are characteristic of white adipocytes. The data suggest that beige adipocytes adjust their production of IL-6 to reach an optimal level for differentiation in the medium enhancing browning in an autocrine manner.

Item Type: Article
Uncontrolled Keywords: OBESITY; Interleukin-6; THERMOGENESIS; Beige adipocyte; Batokine;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
R Medicine / orvostudomány > RZ Other systems of medicine / orvostudomány egyéb területei
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 15 Sep 2019 12:31
Last Modified: 15 Sep 2019 12:31
URI: http://real.mtak.hu/id/eprint/99400

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