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Rapid decline of bacterial drug-resistance in an antibiotic-free environment through phenotypic reversion

Dunai, Anett and Spohn, Réka and Farkas, Zoltán and Lázár, Viktória and Györkei, Ádám and Apjok, Gábor and Boross, Gábor and Szappanos, Balázs and Grézal, Gábor and Faragó, Anikó and Papp, Balázs and Pál, Csaba (2019) Rapid decline of bacterial drug-resistance in an antibiotic-free environment through phenotypic reversion. ELIFE, 8. ISSN 2050-084X

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Abstract

Antibiotic resistance typically induces a fitness cost that shapes the fate of antibiotic-resistant bacterial populations. However, the cost of resistance can be mitigated by compensatory mutations elsewhere in the genome, and therefore the loss of resistance may proceed too slowly to be of practical importance. We present our study on the efficacy and phenotypic impact of compensatory evolution in Escherichia coli strains carrying multiple resistance mutations. We have demonstrated that drug-resistance frequently declines within 480 generations during exposure to an antibiotic-free environment. The extent of resistance loss was found to be generally antibiotic-specific, driven by mutations that reduce both resistance level and fitness costs of antibiotic-resistance mutations. We conclude that phenotypic reversion to the antibiotic-sensitive state can be mediated by the acquisition of additional mutations, while maintaining the original resistance mutations. Our study indicates that restricting antimicrobial usage could be a useful policy, but for certain antibiotics only.

Item Type: Article
Additional Information: Funding Agency and Grant Number: H2020 European Research Council [H2020-ERC-2014-CoG 648364]; Gazdasagfejlesztesi es Innovacios Operativ Programm [GINOP-2.3.2-15-2016-00014, GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-2016-00026]; Hungarian Academy of Sciences Momentum Programme [LP2017-10/2017]; National Research, Development and Innovation Office Elvonal Programme [KKP 126506]; Wellcome Trust [WT 098016/Z/11/Z, WT 084314/Z/07/Z]; National Research, Development and Innovation Office [FK 128775, NKFI-112294]; Magyar Tudomanyos Akademia Lendulet Programme [LP 2012-32/2018]; Magyar Tudomanyos Akademia Postdoctoral Programme [PD-007/2016, PD-038/2015, LP2009-013/2012] Funding text: H2020 European Research Council H2020-ERC-2014-CoG 648364 Resistance Evolution Csaba Pal; Gazdasagfejlesztesi es Innovacios Operativ Programm GINOP-2.3.2-15-2016-00014 Csaba Pal; Gazdasagfejlesztesi es Innovacios Operativ Programm GINOP-2.3.2-15-2016-00020 Csaba Pal; Hungarian Academy of Sciences Momentum Programme LP2017-10/2017 Csaba Pal; National Research, Development and Innovation Office Elvonal Programme KKP 126506 Csaba Pal; Wellcome Trust WT 098016/Z/11/Z Balazs Papp; Gazdasagfejlesztesi es Innovacios Operativ Programm GINOP-2.3.2-15-2016-00026 Balazs Papp; Wellcome Trust WT 084314/Z/07/Z Csaba Pal; National Research, Development and Innovation Office FK 128775 Zoltan Farkas; Magyar Tudomanyos Akademia Lendulet Programme LP 2012-32/2018 Csaba Pal; Magyar Tudomanyos Akademia Postdoctoral Programme PD-007/2016 Viktoria Lazar; Magyar Tudomanyos Akademia Postdoctoral Programme PD-038/2015 Zoltan Farkas; National Research, Development and Innovation Office NKFI-112294 Laszlo Bodai; Magyar Tudomanyos Akademia Lendulet Programme LP2009-013/2012 Balazs Papp; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Uncontrolled Keywords: EVOLUTION; Antibiotic resistance; E. coli; evolutionary biology; compensatory mutations;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 21 Nov 2019 13:50
Last Modified: 21 Nov 2019 13:50
URI: http://real.mtak.hu/id/eprint/103506

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