Monitoring α-Synuclein Proteotoxicity in Drosophila Models.

Szabó, Áron and Tofaris, George K. (2019) Monitoring α-Synuclein Proteotoxicity in Drosophila Models. METHODS IN MOLECULAR BIOLOGY, 1948. pp. 199-208. ISSN 1064-3745

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Official URL: http://doi.org/10.1007/978-1-4939-9124-2_15

Abstract

Parkinson's disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.

Item Type:	Article
Additional Information:	Tim Bartels (ed.), Alpha-Synuclein: Methods and Protocols, Methods in Molecular Biology, vol. 1948, https://doi.org/10.1007/978-1-4939-9124-2_15, © Springer Science+Business Media, LLC, part of Springer Nature 2019
Uncontrolled Keywords:	Neurotoxicity; BEHAVIOR; DROSOPHILA; In vivo models; Morphology analysis;
Subjects:	Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor:	MTMT SWORD
Depositing User:	MTMT SWORD
Date Deposited:	23 Nov 2019 09:51
Last Modified:	23 Nov 2019 09:51
URI:	http://real.mtak.hu/id/eprint/103672

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