Application of overpressured layer chromatography combined with digital autoradiography and mass spectrometry in the study of deramciclane metabolism

Ludányi, Krisztina and Vékey, Károly and Szúnyog, J. and Mincsovics, E. and Karancsi, T. and Klebovich, Imre (1999) Application of overpressured layer chromatography combined with digital autoradiography and mass spectrometry in the study of deramciclane metabolism. JOURNAL OF AOAC INTERNATIONAL, 82 (2). pp. 231-238. ISSN 1060-3271

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Abstract

Overpressured layer chromatography was combined with the highly sensitive and rapid digital autoradiography (DAR) and mass spectrometry to separate, detect, and identify H-3- and C-14-labeled deramciclane metabolites in different biological matrixes. Several minor and major metabolites were separated from plasma and urine samples. The radioactive metabolites localized by DAR were scraped from the thin-layer chromatographic plate and transferred to a mass spectrometer for structure identification. Several metabolites were isolated and characterized, including hydroxy-N-desmethyl deramciclane, which is described in detail. The combination of techniques is efficient and has good sensitivity: about 2 mu g metabolite from a biological matrix was isolated and identified this way.

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