Szigeti, Krisztián and Horváth, Ildikó and Veres, Dániel and Martinecz, Bernadett and Lénárt, Nikolett and Máthé, Domokos and Dénes, Ádám (2015) A novel SPECT-based approach reveals early mechanisms of central and peripheral inflammation after cerebral ischemia. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 35 (12). pp. 1921-1929. ISSN 0271-678X
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Abstract
Inflammation that develops in the brain and peripheral organs after stroke contributes profoundly to poor outcome of patients. However, mechanisms through which inflammation impacts on brain injury and overall outcome are improperly understood, in part because the earliest inflammatory events after brain injury are not revealed by current imaging tools. Here, we show that single-photon emission computed tomography (NanoSPECT/CT Plus) allows visualization of blood brain barrier (BBB) injury after experimental stroke well before changes can be detected with magnetic resonance imaging (MRI). Early 99mTc-DTPA (diethylene triamine pentaacetic acid) signal changes predict infarct development and systemic inflammation preceding experimental stroke leads to very early perfusion deficits and increased BBB injury within 2 hours after the onset of ischemia. Acute brain injury also leads to peripheral inflammation and immunosuppression, which contribute to poor outcome of stroke patients. The SPECT imaging revealed early (within 2 hours) changes in perfusion, barrier function and inflammation in the lungs and the gut after experimental stroke, with good predictive value for the development of histopathologic changes at later time points. Collectively, visualization of early inflammatory changes after stroke could open new translational research avenues to elucidate the interactions between central and peripheral inflammation and to evaluate in vivo 'multi-system' effects of putative anti-inflammatory treatments.Journal of Cerebral Blood Flow & Metabolism advance online publication, 29 July 2015; doi:10.1038/jcbfm.2015.174.
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| Additional Information: | Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Budapest, 1083, Hungary CROmed Translational Research Centers, Budapest, Hungary Cited By :13 Export Date: 21 July 2019 CODEN: JCBMD Correspondence Address: Dénes, Á.; Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Hungary; email: denesa@koki.hu Chemicals/CAS: pentetate technetium tc 99m, 65454-61-7; Radiopharmaceuticals; Technetium Tc 99m Pentetate Tradenames: NanoSPECT/CT PLUS, Mediso Manufacturers: Mediso Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Budapest, 1083, Hungary CROmed Translational Research Centers, Budapest, Hungary Cited By :13 Export Date: 27 August 2019 CODEN: JCBMD Correspondence Address: Dénes, Á.; Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Hungary; email: denesa@koki.hu Chemicals/CAS: pentetate technetium tc 99m, 65454-61-7; Radiopharmaceuticals; Technetium Tc 99m Pentetate Tradenames: NanoSPECT/CT PLUS, Mediso Manufacturers: Mediso Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Budapest, 1083, Hungary CROmed Translational Research Centers, Budapest, Hungary Cited By :13 Export Date: 29 November 2019 CODEN: JCBMD Correspondence Address: Dénes, Á.; Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Hungary; email: denesa@koki.hu Chemicals/CAS: pentetate technetium tc 99m, 65454-61-7; Radiopharmaceuticals; Technetium Tc 99m Pentetate Tradenames: NanoSPECT/CT PLUS, Mediso Manufacturers: Mediso Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Budapest, 1083, Hungary CROmed Translational Research Centers, Budapest, Hungary Cited By :14 Export Date: 30 March 2020 CODEN: JCBMD Correspondence Address: Dénes, Á.; Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Hungary; email: denesa@koki.hu Chemicals/CAS: pentetate technetium tc 99m, 65454-61-7; Radiopharmaceuticals; Technetium Tc 99m Pentetate Tradenames: NanoSPECT/CT PLUS, Mediso Manufacturers: Mediso Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Budapest, 1083, Hungary CROmed Translational Research Centers, Budapest, Hungary Cited By :15 Export Date: 29 July 2020 CODEN: JCBMD Correspondence Address: Dénes, Á.; Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Hungary; email: denesa@koki.hu Chemicals/CAS: pentetate technetium tc 99m, 65454-61-7; Radiopharmaceuticals; Technetium Tc 99m Pentetate Tradenames: NanoSPECT/CT PLUS, Mediso Manufacturers: Mediso Funding details: Hungarian Scientific Research Fund, OTKA, 109743 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Budapest, 1083, Hungary CROmed Translational Research Centers, Budapest, Hungary Cited By :15 Export Date: 31 July 2020 CODEN: JCBMD Correspondence Address: Dénes, Á.; Laboratory of Neuroimmunology, Institute of Experimental Medicine, Szigony u. 43, IX. Tuzoltó u. 37-47, Hungary; email: denesa@koki.hu Chemicals/CAS: pentetate technetium tc 99m, 65454-61-7; Radiopharmaceuticals; Technetium Tc 99m Pentetate Tradenames: NanoSPECT/CT PLUS, Mediso Manufacturers: Mediso Funding details: Hungarian Scientific Research Fund, OTKA, 109743 |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 23 Sep 2020 07:45 |
| Last Modified: | 23 Sep 2020 07:45 |
| URI: | http://real.mtak.hu/id/eprint/114119 |
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