REAL

Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes

Gábor, Erika and Cinege Gyöngyi, Ilona and Csordás, Gábor and Rusvai, Miklós and Honti, Viktor and Török, Tibor and Kurucz, Judit Éva and Andó, István (2020) Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 109. ISSN 0145-305X

[img]
Preview
Text
GaborDevCompImmunol.pdf

Download (1MB) | Preview

Abstract

Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.

Item Type: Article
Uncontrolled Keywords: Immunity; monoclonal antibody; Hemocyte; Apis mellifera; Honey bee; insect immunity;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 28 Sep 2020 06:57
Last Modified: 28 Sep 2020 06:58
URI: http://real.mtak.hu/id/eprint/115016

Actions (login required)

Edit Item Edit Item