REAL

RING-Type E3 Ubiqitin Ligase BarleyGenes(HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components

Zombori, Zoltán and Nagy, Bettina and Mihály, Róbert and Pauk, János and Cseri, András and Sass, László and Horváth V., Gábor and Dudits, Dénes (2020) RING-Type E3 Ubiqitin Ligase BarleyGenes(HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components. PLANTS, 9 (12). ISSN 2223-7747

[img]
Preview
Text
plants-09-01693-v2.pdf

Download (4MB) | Preview

Abstract

Previously, studies on RING-type E3 ubiquitin ligases in cereals were preferentially focused on GW2 genes primarily controlling seed parameters in rice and wheat. Here we report cloning two HvYrg genes from barley that share significant homology with rice GW2 gene. In antisense genotypes efficiency of gene silencing varied between genes and transgenic lines: ASHvYrg1: 30-50% and ASHvYrg2: 20-27%. Reduced activity of both genes altered shoot system with increasing number of side shoots. Changes in leaf width, weight, or plant weight and height reached significant levels in some transgenic lines. Lowering expression of the two barley HvYrg genes caused opposite responses in spike development. Plants with ASHvYrg1 gene construct showed earlier heading time and prolonged grain-filling period, while plants from ASHvYrg2 genotype flowered in delay. Digital imaging of root development revealed that down-regulation of HvYrg1 gene variant stimulated root growth, while ASHvYrg2 plants developed reduced root system. Comparison of seed parameters indicated an increase in thousand grain weight accompanied with longer and wider seed morphology. In summary we conclude that in contrast to inhibition of GW2 genes in rice and wheat plants, down-regulation of the barely HvYrg genes caused substantial changes in vegetative organs in addition to alteration of seed parameters.

Item Type: Article
Subjects: Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 29 Jan 2021 14:26
Last Modified: 29 Jan 2021 14:26
URI: http://real.mtak.hu/id/eprint/120252

Actions (login required)

Edit Item Edit Item