Hakkel, Erzsébet and Varga, Edina and Kovács, Balázs and Stiftné Szilvásy-Szabó, Anett and Cote-Vélez, Antonieta and Péterfi, Zoltán Attila and Tóth, Mónika and Zelena, Dóra and Mezriczky, Zsolt and Kádár, Andrea and Kővári, Dóra and Rózsa J., Balázs and Ruska, Yvette Magdolna and Tóth, Blanka and Máté, Zoltán and Erdélyi, Ferenc and Szabó, Gábor and Gereben, Balázs and Fekete, Csaba (2020) A glial-neuronal circuit in the median eminence regulates thyrotropin-releasing hormone-release via the endocannabinoid system. ISCIENCE, 23 (3). ISSN 2589-0042
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A-GlialNeuronal-Circuit-in-the-Median-Eminence-Regulates-ThyrotropinReleasing-HormoneRelease-via-the-Endocannabinoid-System2020iScienceOpen-Access.pdf Download (3MB) | Preview |
Official URL: http://doi.org/10.1016/j.isci.2020.100921
| Item Type: | Article |
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| Additional Information: | Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest, 1083, Hungary Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, 62210, Mexico Department of Chemistry, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China Department of Behavioral Neurobiology, Institute of Experimental Medicine, Budapest, Hungary Centre for Neuroscience, Szentágothai Research Centre, Institute of Physiology, Medical School, University of Pécs, Pécs, 7624, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, 1088, Hungary Department of Anatomy, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-0033, Japan Gill Center for Biomolecular Science, Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 474052, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Budapest, 1083, Hungary Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellert ter 4, Budapest, 1111, Hungary Medical Gene Technology Unit, Institute of Experimental Medicine, Budapest, 1083, Hungary Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, Tupper Research Institute, Tufts Medical Center, Boston, MA 02111, United States Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, United States Cited By :1 Export Date: 7 March 2021 Correspondence Address: Fekete, C.; Department of Endocrine Neurobiology, Szigony u. 43, Hungary; email: fekete.csaba@koki.mta.hu Funding details: Budapesti Műszaki és Gazdaságtudományi Egyetem, BME Funding details: Horizon 2020 Framework Programme, H2020, 666869 Funding details: Consejo Nacional de Ciencia y Tecnología, CONACYT, CB2015/254960, ERC682426, GINOP-2016-00979, KFI-2016-0177, NVKP-2016-0043 Funding details: Hungarian Scientific Research Fund, OTKA, K109710 Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding details: Hungarian Science Foundation Funding text 1: This work was supported by Grants from the Hungarian Science Foundation (OTKA K109710), the Hungarian National Brain Research Program (2017-1.2.1-NKP-2017-00002), EU H2020 THYRAGE no. 666869, CONACYT-Mexico (CB2015/254960), ERC682426, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043 and by the BME-Biotechnology FIKP grant of EMMI (BME FIKP-BIO). The authors express their gratitude to Zs?fia L?szl? for her help during the preparing of some of the confocal microscopic images. E.F. performed the immunofluorescent and ultrastructural studies. E.V. and B.K. designed and performed the patch clamp experiments. B.K. performed the optogenetic experiments. Z.P. deigned and performed the calcium imaging experiments. A.C.-V. J.-L.C. P.J.-B. A.S.-S. and Y.R. designed and performed the ME explant experiments and analyzed these data. M.M. synthesized, purified, and chemically characterized P-TRH. B.T. performed the 2-AG measurement. M.T. performed and analyzed the in situ hybridization experiments. F.E. Z.M. G. S. and B.G. were involved in the generation of TRH-IRES-Cre mice. A.S.-S. and D.K. isolated the tanycytes and performed the gene expression analysis. D.Z. performed the stereotaxic AAV injections. A.K. performed immunostaining. Zs.M. and B.R. helped to design and perform the calcium imaging experiments and analyzed data. M.W. provided CB1 antibody. M.K. provided tissues of DAGL? KO mice. K.M. provided DAGL? antibody. R.M.L. analyzed data and wrote the manuscript. C.F. acquired the funding, conceptualized and interpreted the studies, and wrote the manuscript. B.R. is one of the founders of Femtonics and is a member of its scientific advisory board. The other authors have declared that no conflict of interest exists. Funding text 2: This work was supported by Grants from the Hungarian Science Foundation ( OTKA K109710 ), the Hungarian National Brain Research Program ( 2017-1.2.1-NKP-2017-00002 ), EU H2020 THYRAGE no. 666869, CONACYT -Mexico ( CB2015/254960 ), ERC682426 , KFI-2016-0177 , GINOP-2016-00979 , NVKP-2016-0043 and by the BME-Biotechnology FIKP grant of EMMI (BME FIKP-BIO). The authors express their gratitude to Zsófia László for her help during the preparing of some of the confocal microscopic images. |
| Subjects: | R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 12 Mar 2021 10:05 |
| Last Modified: | 12 Mar 2021 10:05 |
| URI: | http://real.mtak.hu/id/eprint/122192 |
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