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A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses

Holderith, Noémi and Herédi, Judit and Kis, Viktor and Nusser, Zoltán (2020) A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. CELL REPORTS, 32 (4). ISSN 2211-1247

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Abstract

Elucidating the molecular mechanisms underlying the functional diversity of synapses requires a high-resolution, sensitive, diffusion-free, quantitative localization method that allows the determination of many proteins in functionally characterized individual synapses. Array tomography permits the quantitative analysis of single synapses but has limited sensitivity, and its application to functionally characterized synapses is challenging. Here, we aim to overcome these limitations by searching the parameter space of different fixation, resin, embedding, etching, retrieval, and elution conditions. Our optimizations reveal that etching epoxy-resin-embedded ultrathin sections with Na-ethanolate and treating them with SDS dramatically increase the labeling efficiency of synaptic proteins. We also demonstrate that this method is ideal for the molecular characterization of individual synapses following paired recordings, two-photon [Ca2+] or glutamate-sensor (iGluSnFR) imaging. This method fills a missing gap in the toolbox of molecular and cellular neuroscience, helping us to reveal how molecular heterogeneity leads to diversity in function.

Item Type: Article
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 16 Mar 2021 08:56
Last Modified: 16 Mar 2021 08:56
URI: http://real.mtak.hu/id/eprint/122416

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