Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells

Péntek, Adrienn and Pászty, Katalin and Apáti, Ágota (2014) Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells. METHODS IN MOLECULAR BIOLOGY. pp. 1-11. ISSN 1064-3745

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Measurement of changes in intracellular calcium concentration is one of the most common and useful tools for studying signal transduction pathways or cellular responses in basic research and drug screening purposes as well. Increasing number of such applications using human pluripotent stem cells and their derivatives requires development of calcium signal measurements for this special cell type. Here we describe a modified protocol for analysis of calcium signaling events in human embryonic stem cells, which can be used for other pluripotent cell types (such as iPSC) or their differentiated offspring as well.

Item Type: Article
Uncontrolled Keywords: human embryonic stem cell, calcium signaling, fluorescent calcium indicators, genetically encoded calcium indicators, cocnfocal microscopy
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: András Bognár
Date Deposited: 16 Jun 2014 09:09
Last Modified: 16 Jun 2014 09:09

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