Wirth, Roland and Pap, Bernadett and Dudits, Dénes and Kakuk, Balázs and Bagi, Zoltán and Shetty, Prateek and Kovács, Kornél Lajos and Maróti, Gergely (2021) Genome-centric investigation of anaerobic digestion using sustainable second and third generation substrates. JOURNAL OF BIOTECHNOLOGY, 339. pp. 53-64. ISSN 0168-1656
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Abstract
Biogas production through co-digestion of second and third generation substrates is an environmentally sustainable approach. Green willow biomass, chicken manure waste and microalgae biomass substrates were combined in the anaerobic digestion experiments. Biochemical methane potential test showed that biogas yields of co-digestions were significantly higher compared to the yield when energy willow was the sole substrate. To scale up the experiment continuous stirred-tank reactors (CSRTs) are employed, digestion parameters are monitored. Furthermore, genome-centric metagenomics approach was employed to gain functional insight into the complex anaerobic decomposing process. This revealed the importance of Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes phyla as major bacterial participants, while Methanomicrobia and Methanobacteria represented the archaeal constituents of the communities. The bacterial phyla were shown to perform the carbohydrate hydrolysis. Among the representatives of long-chain carbohydrate hydrolysing microbes Bin_61: Clostridia is newly identified metagenome assembled genome (MAG) and Bin_13: DTU010 sp900018335 is common and abundant in all CSTRs. Methanogenesis was linked to the slow-growing members of the community, where hydrogenotrophic methanogen species Methanoculleus (Bin_10) and Methanobacterium (Bin_4) predominate. A sensitive balance between H2 producers and consumers was shown to be critical for stable biomethane production and efficient waste biodegradation.
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| Additional Information: | Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Department of Biotechnology, University of Szeged, Szeged, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Faculty of Water Sciences, University of Public Service, Baja, Hungary Export Date: 30 August 2021 CODEN: JBITD Correspondence Address: Maróti, G.; Institute of Plant Biology, Hungary; email: maroti.gergely@brc.hu Funding Agency and Grant Number: Hungarian National Research, Development and Innovation Fund [GINOP-2.2.1-15-2017-00081, 2020-1.1.2-PIACI-KFI-2020-00117, EFOP-3.6.2-16-2017-00010]; Hungarian NKFIH fundNational Research, Development & Innovation Office (NRDIO) - Hungary [PD132145, FK123902, FK123899]; Lendulet-Programme (GM) of the Hungarian Academy of Sciences [LP2020-5/2020] Funding text: This study has been supported in part by the Hungarian National Research, Development and Innovation Fund projects GINOP-2.2.1-15-2017-00081, EFOP-3.6.2-16-2017-00010 and 2020-1.1.2-PIACI-KFI-2020-00117. RW, BZ and GM received support from the Hungarian NKFIH fund projects PD132145, FK123902 and FK123899. This work was also supported by the Lendulet-Programme (GM) of the Hungarian Academy of Sciences (LP2020-5/2020) . |
| Uncontrolled Keywords: | Biogas; CO-DIGESTION; Microalgal biomass; Waste Biomasses; metagenomics; willow shrub; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia Q Science / természettudomány > QK Botany / növénytan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 06 Dec 2021 16:13 |
| Last Modified: | 06 Dec 2021 16:13 |
| URI: | http://real.mtak.hu/id/eprint/134246 |
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