REAL

PROTEIN CONFORMATION MONITORED BY ENERGY-SELECTIVE OPTICAL SPECTROSCOPY

VANDERKOOI, J. M. and Kaposi, András Dezső and FIDY, J. (1993) PROTEIN CONFORMATION MONITORED BY ENERGY-SELECTIVE OPTICAL SPECTROSCOPY. TRENDS IN BIOCHEMICAL SCIENCES, 18 (3). pp. 71-76. ISSN 0968-0004

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Abstract

Fluctuations in the polypeptide chain lead to disorder in proteins and to a distribution in the parameters that regulate their functions. Using low temperature (to reduce the fluctuations) and narrow-band lasers (to select one substate among the many forms), high-resolution absorption and fluorescence spectra for chromophores in proteins can be obtained. These spectra reveal information on the kind and extent of disorder in proteins and allow for the determination of the vibrational energies of both ground and excited state molecules, true inhomogeneous spectral width, and kinetic studies of individual protein substates.

Item Type: Article
Subjects: Q Science / természettudomány > QC Physics / fizika
Q Science / természettudomány > QD Chemistry / kémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 09 Jul 2014 12:09
Last Modified: 06 Oct 2015 18:44
URI: http://real.mtak.hu/id/eprint/13630

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