Peirsman, Arne and Blondeel, Eva and Ahmed, Tasdiq and Anckaert, Jasper and Audenaert, Dominique and Buzás, Krisztina and Diósdi, Ákos and Horváth, Péter (2021) MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity. NATURE METHODS, 18 (11). pp. 1294-1303. ISSN 1548-7091
|
Text
s41592-021-01291-4.pdf Available under License Creative Commons Attribution. Download (11MB) | Preview |
Abstract
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Agency and Grant Number: Concerted Research Actions from Ghent University [01G02221]; Stichting Tegen Kanker [2020-073]; Kom Op Tegen Kanker (Stand up to Cancer); Flemish cancer society; Fund for Scientific Research FlandersFWO [1198121N]; EU Horizon2020 project LSFM4LIFE [668350-2]; EU FET-OPEN project BRIGHTER [828931]; ZonMw-BMBF - Onconoid Hub [114027003]; Cancer Research UKCancer Research UK [A28596, A29368]; The Brain Tumour Charity [GN-000676]; US National Institutes of Health (NIH)United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 CA196018, R21 AG061687]; Sigrid Juseliuksen Foundation; Cancer Society of Finland; Canadian Institutes of Health Research (CIHR)Canadian Institutes of Health Research (CIHR) [147081]; German Federal Ministry of Education and Research ((BMBF))Federal Ministry of Education & Research (BMBF) [13N11115, 03Z1N512] Funding text: This work was supported by the Concerted Research Actions from Ghent University (grant no. 01G02221), Stichting Tegen Kanker (grant no. 2020-073), Kom Op Tegen Kanker (Stand up to Cancer), the Flemish cancer society, and Fund for Scientific Research Flanders (grant no. 1198121N) (O.D.W.); EU Horizon2020 project LSFM4LIFE (grant no. 668350-2), the EU FET-OPEN project BRIGHTER (grant no. 828931) and the ZonMw-BMBF joint sponsored project Onconoid Hub (grant no. 114027003) (F.Pa.); Cancer Research UK (grant no. A28596 and A29368) and The Brain Tumour Charity (grant no. GN-000676) (N.C.); US National Institutes of Health (NIH) (grant no. R01 CA196018 and R21 AG061687) (S.T.); Sigrid Juseliuksen Foundation and Cancer Society of Finland (J.H.); Canadian Institutes of Health Research (CIHR) (grant no. 147081) (S.M.R.); German Federal Ministry of Education and Research ((BMBF), grant no. 13N11115 (ProMEBS)) (P.S.); and German Federal Ministry of Education and Research ((BMBF), grant no. 03Z1N512) (L.A.K.-S., S.Mi.). |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 07 Feb 2022 12:17 |
| Last Modified: | 07 Feb 2022 12:17 |
| URI: | http://real.mtak.hu/id/eprint/137537 |
Actions (login required)
 |
Edit Item |
