Role of ethylene in ER stress and the unfolded protein response in tomato (Solanum lycopersicum L.) plants

Czékus, Zalán and Szalai, Gabriella and Görgényi Miklósné Tari, Irma and Khan, M. Iqbal R. and Poór, Péter (2022) Role of ethylene in ER stress and the unfolded protein response in tomato (Solanum lycopersicum L.) plants. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 181. pp. 1-11. ISSN 0981-9428

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Abstract

The unfolded protein response (UPR) plays a significant role in the maintenance of cellular homeostasis under endoplasmic reticulum (ER) stress, which is highly dependent on the regulation of defense-related phytohor- mones. In this study, the role of ethylene (ET) in ER stress and UPR was investigated in the leaves of intact tomato (Solanum lycopersicum) plants. Exogenous application of the ET precursor 1-aminocyclopropane-1-car- boxylic acid not only resulted in higher ET emission from leaves but also increased the expression of the UPR marker gene SlBiP and the transcript levels of the ER stress sensor SlIRE1, as well as the levels of SlbZIP60, after 24 h in tomato leaves. Using ET receptor Never ripe (Nr) mutants, a significant role of ET in tunicamycin (Tm)- induced ER stress sensing and signaling was confirmed based on the changes in the expression levels of SlIRE1b and SlBiP. Furthermore, the analysis of other defense-related phytohormones showed that the Tm-induced ET can affect positively the levels of and response to salicylic acid. Additionally, it was found that nitric oxide pro- duction and lipid peroxidation, as well as the electrolyte leakage induced by Tm, is regulated by ET, whereas the levels of H2O2 and proteolytic activity seemed to be independent of ET under ER stress in the leaves of tomato plants.

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