Leptin promotes a pro-inflammatory lipid profile and induces inflammatory pathways in human SZ95 sebocytes.

Törőcsik, D. and Kovács, D. and Camera, E. and Lovászi, M. and Cseri, K. and Nagy, G. G. and Molinaro, R. and Rühl, R. and Tax, G. and Szabó, K. and Picardo, M. and Kemény, L. and Zouboulis, C. C. and Remenyik, E. (2014) Leptin promotes a pro-inflammatory lipid profile and induces inflammatory pathways in human SZ95 sebocytes. The British journal of dermatology. ISSN 1365-2133


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BACKGROUND Leptin, the adipocyte-secreted hormone that regulates weight, is known to link lipid metabolism with inflammation in various cell types, however its role in human sebocytes has not yet been investigated. OBJECTIVES The purpose of the study was to investigate the effects of leptin in human sebaceous gland biology. METHODS Ob-Rb expression was detected by real time quantitative RT-PCR and by immunochemistry. Lipid analysis was performed by high performance thin layer chromatography, gas-chromatography-mass spectrometry and time of flight mass spectrometer mass detection. Lipid bodies were visualized by BODIPY staining using fluorescent microscopy and measured by flow cytometry. IL-6 and IL-8 mRNA levels were assessed by real time quantitative RT-PCR and their release was evaluated by enzyme-linked immunosorbent assay. COX-2 and 5-LOX protein expressions as well as the phosphorylation of p65 and STAT3 were determined by Western blot analysis. RESULTS Expression of Ob-Rb was detected in human sebaceous glands and in cultured human SZ95 sebocytes. The treatment of SZ95 sebocytes with leptin led to an enlargement of intracellular lipid bodies, increased the ratios of unsaturated/saturated fatty acids and decreased vitamin E levels. Further supporting a pro-inflammatory role, leptin induced COX-2 and 5-LOX expression in SZ95 sebocytes and augmented the production of IL-6 and IL-8 cytokines. Upon leptin treatment, STAT-3 and NF-κB pathways were activated indicating that these known leptin signalling pathways are active in human sebocytes. CONCLUSIONS Our findings suggest that leptin signalling may be involved in the pro-inflammatory regulation of sebaceous lipid metabolism and in the induction of inflammatory enzymes and cytokines. This article is protected by copyright. All rights reserved.

Item Type: Article
Subjects: R Medicine / orvostudomány > RL Dermatology / bőrgyógyászat
Depositing User: Dr Daniel Torocsik
Date Deposited: 17 Sep 2014 12:51
Last Modified: 17 Sep 2014 12:51

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