Vanilloid receptor-1 (TRPV1) expression and function in the vasculature of the rat

Tóth, Attila and Czikora, Ágnes and Pásztorné Tóth, Enikő and Dienes, Beatrix and Bay, Péter and Csernoch, László and Rutkai, Ibolya and Csató, Viktória and Mányiné Siket, Ivetta and Pórszász, Róbert and Édes, István and Papp, Zoltán and Boczán, Judit (2014) Vanilloid receptor-1 (TRPV1) expression and function in the vasculature of the rat. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 62 (2). pp. 129-144. ISSN 0022-1554

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Transient receptor potential (TRP) cation channels are emerging in vascular biology. In particular, the expression of the capsaicin receptor (TRPV1) was reported in vascular smooth muscle cells. This study characterized the arteriolar TRPV1 function and expression in the rat. TRPV1 mRNA was expressed in various vascular beds. Six commercially available antibodies were tested for TRPV1 specificity. Two of them were specific (immunostaining was abolished by blocking peptides) for neuronal TRPV1 and one recognized vascular TRPV1. TRPV1 was expressed in blood vessels in the skeletal muscle, mesenteric and skin tissues, as well as in the aorta and carotid arteries. TRPV1 expression was found to be regulated at the level of individual blood vessels, where some vessels expressed, while others did not express TRPV1 in the same tissue sections. Capsaicin (a TRPV1 agonist) evoked constrictions in skeletal muscle arteries and in the carotid artery, but had no effect on the femoral and mesenteric arteries or the aorta. In blood vessels, TRPV1 expression was detected in most of the large arteries, but there were striking differences at level of the small arteries. TRPV1 activity was suppressed in some isolated arteries. This tightly regulated expression and function suggests a physiological role for vascular TRPV1.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia
Depositing User: MTMT SWORD
Date Deposited: 17 Sep 2014 09:19
Last Modified: 17 Sep 2014 09:19

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