Organelle-specific localization of glutathione in plants grown under different light intensities and spectra

Gasperl, A. and Zellnig, G. and Kocsy, Gábor and Müller, M. (2022) Organelle-specific localization of glutathione in plants grown under different light intensities and spectra. HISTOCHEMISTRY AND CELL BIOLOGY, 158. pp. 213-227. ISSN 0948-6143

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Plant ascorbate and glutathione metabolism counteracts oxidative stress mediated, for example, by excess light. In this review, we discuss the properties of immunocytochemistry and transmission electron microscopy, redox-sensitive dyes or probes and bright-field microscopy, confocal microscopy or fluorescence microscopy for the visualization and quantification of glutathione at the cellular or subcellular level in plants and the quantification of glutathione from isolated organelles. In previous studies, we showed that subcellular ascorbate and glutathione levels in Arabidopsis are affected by high light stress. The use of light-emitting diodes (LEDs) is gaining increasing importance in growing indoor crops and ornamental plants. A combination of different LED types allows custom-made combinations of wavelengths and prevents damage related to high photon flux rates. In this review we provide an overview on how different light spectra and light intensities affect glutathione metabolism at the cellular and subcellular levels in plants. Findings obtained in our most recent study demonstrate that both light intensity and spectrum significantly affected glutathione metabolism in wheat at the transcriptional level and caused genotype-specific reactions in the investigated Arabidopsis lines. © 2022, The Author(s).

Item Type: Article
Additional Information: Institute of Biology, Plant Sciences, NAWI Graz, University of Graz, Graz, 8010, Austria Agricultural Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Martonvásár, 2462, Hungary Export Date: 9 June 2022 CODEN: HCBIF Correspondence Address: Müller, M.; Institute of Biology, Austria; email:
Uncontrolled Keywords: REDOX STATE; Immunolabeling; roGFP; Oxidative stress; Light intensity and spectrum; Subcellular glutathione content in plants;
Subjects: Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan
S Agriculture / mezőgazdaság > SB Plant culture / növénytermesztés
Depositing User: MTMT SWORD
Date Deposited: 01 Mar 2023 16:27
Last Modified: 01 Mar 2023 16:27

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