An Allosteric Signaling Pathway of Human 3- Phosphoglycerate Kinase from Force Distribution Analysis

Palmai, Zoltan and Seifert, Christian and Grater, Frauke and Balog, Erika (2014) An Allosteric Signaling Pathway of Human 3- Phosphoglycerate Kinase from Force Distribution Analysis. PLOS COMPUTATIONAL BIOLOGY, 10 (1). e1003444. ISSN 1553-734X

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3-Phosphogycerate kinase (PGK) is a two domain enzyme, which transfers a phosphate group between its two substrates, 1,3-bisphosphoglycerate bound to the N-domain and ADP bound to the C-domain. Indispensable for the phosphoryl transfer reaction is a large conformational change from an inactive open to an active closed conformation via a hinge motion that should bring substrates into close proximity. The allosteric pathway resulting in the active closed conformation has only been partially uncovered. Using Molecular Dynamics simulations combined with Force Distribution Analysis (FDA), we describe an allosteric pathway, which connects the substrate binding sites to the interdomain hinge region. Glu192 of alpha-helix 7 and Gly394 of loop L14 act as hinge points, at which these two secondary structure elements straighten, thereby moving the substrate-binding domains towards each other. The long-range allosteric pathway regulating hPGK catalytic activity, which is partially validated and can be further tested by mutagenesis, highlights the virtue of monitoring internal forces to reveal signal propagation, even if only minor conformational distortions, such as helix bending, initiate the large functional rearrangement of the macromolecule.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: Dr. Erika Balog
Date Deposited: 26 Sep 2014 07:43
Last Modified: 26 Sep 2014 07:43

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