Full-length genome sequence analysis of a Hungarian Porcine Reproductive and Respiratory Syndrome Virus isolated from severe respiratory disease

Bálint, Ádám and Balka, Gyula and Horváth, Péter and Kecskeméti, Sándor and Dán, Ádám and Farsang, Attila and Szeredi, Levente and Bányai, Krisztián and Bartha, Dániel and Olasz, Ferenc and Belák, Sándor and Zádori, Zoltán (2014) Full-length genome sequence analysis of a Hungarian Porcine Reproductive and Respiratory Syndrome Virus isolated from severe respiratory disease. Archives of Virology, 160 (2). pp. 417-22. ISSN 0304-8608

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Abstract

The authors report the isolation of a Type 1 PRRSV strain from a clinical outbreak of severe respiratory problems and high fever. Next generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, containing mostly Spanish sequences and shows highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. SimPlot analysis performed with the available full-length genome sequences indicates no evidences of recombination. Mutation analysis of 9625/2012 and the most related strains revealed high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of the selective immune pressure in the evolution of PRRSV 9625/2012.

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