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Phenotyping shows improved physiological traits and seed yield of transgenic wheat plants expressing the alfalfa aldose reductase under permanent drought stress

Fehér-Juhász, E. and Majer, P. and Sass, László and Lantos, C. and Csiszár, Jolán and Túróczy, Zoltán and Mai, Antal and Horváth V., Gábor and Vass, Imre and Dudits, Dénes (2014) Phenotyping shows improved physiological traits and seed yield of transgenic wheat plants expressing the alfalfa aldose reductase under permanent drought stress. ACTA PHYSIOLOGIAE PLANTARUM, 36. pp. 663-673. ISSN 0137-5881

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Abstract

Members of the aldo-keto reductase family including aldose reductases are involved in antioxidant defense by metabolizing a wide range of lipid peroxidation-derived cytotoxic compounds. Therefore, we produced transgenic wheat genotypes over-expressing the cDNA of alfalfa aldose reductase gene. These plants consequently exhibit 1.5-4.3 times higher detoxification activity for the aldehyde substrate. Permanent drought stress was generated in the greenhouse by growing wheat plants in soil with 20 % water capacity. The control and stressed plants were monitored by a semi automatic phenotyping platform providing computer-controlled watering, digital and thermal imaging. Calculation of biomass values was based on the correlation (R2 = 0.7556) between fresh weight and green pixel-based shoot surface area. The green biomass production by plants of the three transgenic lines was 12-26-41 % higher than the non-transgenic plants' grown under water limitation. Thermal imaging of stressed non-transgenic plants indicated an elevation in the leaf temperature. The thermal status of transformants was similar at both normal and suboptimal water regime. In drought, the transgenic plants used more water during the growing season. The described phenotyping platform provided a comprehensive data set demonstrating the improved physiological condition of the drought stressed transgenic wheat plants in the vegetative growth phase. In soil with reduced water capacity two transgenic genotypes showed higher seed weight per plant than the control non-transgenic one. Limitation of greenhouse-based phenotyping in analysis of yield potential is discussed. © 2013 The Author(s).

Item Type: Article
Uncontrolled Keywords: Water usage; TRANSFORMATION; seed production; Digital and thermal imaging; BIOMASS; Aldo-keto reductase
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH540 Ecology / ökológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 02 Oct 2014 14:09
Last Modified: 03 Oct 2014 12:27
URI: http://real.mtak.hu/id/eprint/17474

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