REAL

The TRANSPLANTA Collection of Arabidopsis Lines: A resource for Functional Analysis of Transcription Factors based on their conditional overexpression

Coego, A. and Brizuela, E. and Castillejo, P. and Ruíz, S. and Koncz, Csaba and Cséplő, Ágnes and Rigó, Gábor and Zsigmond, Laura and Szabados, László (2014) The TRANSPLANTA Collection of Arabidopsis Lines: A resource for Functional Analysis of Transcription Factors based on their conditional overexpression. PLANT JOURNAL, 77 (6). pp. 944-953. ISSN 0960-7412

[img] Text
CoegoPlantJ.pdf
Restricted to Repository staff only

Download (1MB) | Request a copy

Abstract

Transcription factors (TFs) are key regulators of gene expression in all organisms. In eukaryotes, TFs are often represented by functionally redundant members of large gene families. Overexpression might prove a means to unveil the biological functions of redundant TFs; however, constitutive overexpression of TFs frequently causes severe developmental defects, preventing their functional characterization. Conditional overexpression strategies help to overcome this problem. Here, we report on the TRANSPLANTA collection of Arabidopsis lines, each expressing one of 949 TFs under the control of a beta-estradiol-inducible promoter. Thus far, 1636 independent homozygous lines, representing an average of 2.6 lines for every TF, have been produced for the inducible expression of 634 TFs. Along with a GUS-GFP reporter, randomly selected TRANSPLANTA lines were tested and confirmed for conditional transgene expression upon beta-estradiol treatment. As a proof of concept for the exploitation of this resource, beta-estradiol-induced proliferation of root hairs, dark-induced senescence, anthocyanin accumulation and dwarfism were observed in lines conditionally expressing full-length cDNAs encoding RHD6, WRKY22, MYB123/TT2 and MYB26, respectively, in agreement with previously reported phenotypes conferred by these TFs. Further screening performed with other TRANSPLANTA lines allowed the identification of TFs involved in different plant biological processes, illustrating that the collection is a powerful resource for the functional characterization of TFs. For instance, ANAC058 and a TINY/AP2 TF were identified as modulators of ABA-mediated germination potential, and RAP2.10/DEAR4 was identified as a regulator of cell death in the hypocotyl-root transition zone. Seeds of TRANSPLANTA lines have been deposited at the Nottingham Arabidopsis Stock Centre for further distribution.

Item Type: Article
Subjects: Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 02 Oct 2014 14:26
Last Modified: 02 Oct 2014 14:26
URI: http://real.mtak.hu/id/eprint/17478

Actions (login required)

Edit Item Edit Item