Horváth, Beatrix and Güngör, Berivan and Tóth, Mónika and Domonkos, Ágota and Ayaydin, Ferhan and Saifi, Farheen and Chen, Yuhui and Biró, János Barnabás and Bourge, Mickael and Szabó, Zoltán and Tóth, Zoltán and Chen, Rujin and Kaló, Péter (2023) The Medicago truncatula nodule‐specific cysteine‐rich peptides, NCR343 and NCR‐new35 are required for the maintenance of rhizobia in nitrogen‐fixing nodules. NEW PHYTOLOGIST, 239 (5). pp. 1974-1988. ISSN 0028-646X
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Abstract
In the nodules of IRLC legumes, including Medicago truncatula, nitrogen-fixing rhizobia undergo terminal differentiation resulting in elongated and endoreduplicated bacteroids specialized for nitrogen fixation. This irreversible transition of rhizobia is mediated by host produced nodule-specific cysteine-rich (NCR) peptides, of which c. 700 are encoded in the M. truncatula genome but only few of them have been proved to be essential for nitrogen fixation. We carried out the characterization of the nodulation phenotype of three ineffective nitrogen-fixing M. truncatula mutants using confocal and electron microscopy, monitored the expression of defence and senescence-related marker genes, and analysed the bacteroid differentiation with flow cytometry. Genetic mapping combined with microarray- or transcriptome-based cloning was used to identify the impaired genes. Mtsym19 and Mtsym20 mutants are defective in the same peptide NCR-new35 and the lack of NCR343 is responsible for the ineffective symbiosis of NF-FN9363. We found that the expression of NCR-new35 is significantly lower and limited to the transition zone of the nodule compared with other crucial NCRs. The fluorescent protein-tagged version of NCR343 and NCR-new35 localized to the symbiotic compartment. Our discovery added two additional members to the group of NCR genes essential for nitrogen-fixing symbiosis in M. truncatula.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Hungarian National Research Fund/National Research, Development and Innovation Office [OTKA-67576, PD104334/108923, 106068, 119652, 120122/120300, PD-121110, PD-132495]; Collaborative Research Programme ICGEB Research Grant [HUN17-03]; l'Agence Nationale de la Recherche [ANR-11-EQPX-0029/Morphoscope, ANR-10-INBS-04/FranceBioImaging, ANR-11-IDEX-0003-02/Saclay Plant Sciences]; H2020 [739593] Funding text: & nbsp;This work was supported by the Hungarian National Research Fund/National Research, Development and Innovation Office grants OTKA-67576, PD104334/108923, 106068, 119652 and 120122/120300, PD-121110 and PD-132495 as well as by the Collaborative Research Programme ICGEB Research Grant HUN17-03. The present work has benefited from Imagerie-Gif core facility supported by l'Agence Nationale de la Recherche (ANR-11-EQPX-0029/Morphoscope, ANR-10-INBS-04/FranceBioImaging; ANR-11-IDEX-0003-02/Saclay Plant Sciences). FA received funding from H2020 (SGA no. 739593). We thank H. Cs. Tolnaine for her skilful technical assistance and A. Farkas for carrying out sample preparation SEM imaging. |
| Uncontrolled Keywords: | indeterminate nodule, legume, Medicago truncatula, nitrogen-fixing symbiosis, nodule-specific cysteine-rich peptide |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia Q Science / természettudomány > QK Botany / növénytan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 25 Sep 2023 12:27 |
| Last Modified: | 25 Sep 2023 12:27 |
| URI: | http://real.mtak.hu/id/eprint/174830 |
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