Szepesi, Ágnes and Bakacsy, László and Fehér, Attila and Kovács, Henrietta and Pálfi, Péter and Poór, Péter and Szőllősi, Réka and Gondor, Orsolya Kinga and Janda, Tibor and Szalai, Gabriella and Szabados, László and Zsigmond, Laura (2023) L-Aminoguanidine Induces Imbalance of ROS/RNS Homeostasis and Polyamine Catabolism of Tomato Roots after Short-Term Salt Exposure. ANTIOXIDANTS, 12 (8). ISSN 2076-3921
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Abstract
Polyamine (PA) catabolism mediated by amine oxidases is an important process involved in fine-tuning PA homeostasis and related mechanisms during salt stress. The significance of these amine oxidases in short-term responses to salt stress is, however, not well understood. In the present study, the effects of L-aminoguanidine (AG) on tomato roots treated with short-term salt stress induced by NaCl were studied. AG is usually used as a copper amine oxidase (CuAO or DAO) inhibitor. In our study, other alterations of PA catabolism, such as reduced polyamine oxidase (PAO), were also observed in AG-treated plants. Salt stress led to an increase in the reactive oxygen and nitrogen species in tomato root apices, evidenced by in situ fluorescent staining and an increase in free PA levels. Such alterations were alleviated by AG treatment, showing the possible antioxidant effect of AG in tomato roots exposed to salt stress. PA catabolic enzyme activities decreased, while the imbalance of hydrogen peroxide (H2O2), nitric oxide (NO), and hydrogen sulfide (H2S) concentrations displayed a dependence on stress intensity. These changes suggest that AG-mediated inhibition could dramatically rearrange PA catabolism and related reactive species backgrounds, especially the NO-related mechanisms. More studies are, however, needed to decipher the precise mode of action of AG in plants exposed to stress treatments.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: National Research, Development and Innovation (NRDI) Office of the Hungarian Ministry [FK129061, FK128920]; European Union; State of Hungary through the European Social Fund [TAMOP 4.2.4.A/2-11-1-2012-0001]; Institute of Balassi Funding text: This research was funded by the National Research, Development and Innovation (NRDI) Office of the Hungarian Ministry, with grant number FK129061 for A.S and grant number FK128920 for L.Z. Additionally, A.S. gratefully acknowledges the European Union and the State of Hungary for co-financing through the European Social Fund in the framework of TAMOP 4.2.4.A/2-11-1-2012-0001 National Excellence Program' and thanks the Institute of Balassi for the Campus Hungary Scholarships. |
| Subjects: | Q Science / természettudomány > QK Botany / növénytan Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 26 Sep 2023 14:27 |
| Last Modified: | 26 Sep 2023 14:27 |
| URI: | http://real.mtak.hu/id/eprint/175126 |
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