Dendritic spikes induce ripples in parvalbumin interneurons during hippocampal sharp waves.

Chiovini, Balázs and Turi, GF. and Katona, Gergely and Kaszás, Attila and Pálfi, Dénes and Káli, Szabolcs and Rózsa J., Balázs (2014) Dendritic spikes induce ripples in parvalbumin interneurons during hippocampal sharp waves. NEURON, 82 (4). pp. 908-924. ISSN 0896-6273

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Sharp-wave ripples are transient oscillatory events in the hippocampus that are associated with the reactivation of neuronal ensembles within specific circuits during memory formation. Fast-spiking, parvalbumin-expressing interneurons (FS-PV INs) are thought to provide fast integration in these oscillatory circuits by suppressing regenerative activity in their dendrites. Here, using fast 3D two-photon imaging and a caged glutamate, we challenge this classical view by demonstrating that FS-PV IN dendrites can generate propagating Ca(2+) spikes during sharp-wave ripples. The spikes originate from dendritic hot spots and are mediated dominantly by L-type Ca(2+) channels. Notably, Ca(2+) spikes were associated with intrinsically generated membrane potential oscillations. These oscillations required the activation of voltage-gated Na(+) channels, had the same frequency as the field potential oscillations associated with sharp-wave ripples, and controlled the phase of action potentials. Furthermore, our results demonstrate that the smallest functional unit that can generate ripple-frequency oscillations is a segment of a dendrite.

Item Type: Article
Uncontrolled Keywords: Photic Stimulation; Parvalbumins/*metabolism; Mice, Inbred C57BL; Interneurons/*cytology/drug effects/metabolism; Imaging, Three-Dimensional; Hippocampus/*cytology/*physiology; Green Fluorescent Proteins/genetics; Glutamic Acid/pharmacology; Dendrites/drug effects/*physiology; Calcium/metabolism; Brain Waves/drug effects/*physiology; Animals; Action Potentials/drug effects/*physiology
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
Depositing User: MTMT SWORD
Date Deposited: 06 Nov 2014 14:29
Last Modified: 13 Apr 2015 11:56

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