Ellipsometry monitoring of sensor processes based on gold nanoparticle bonded proteins

Lábadi, Zoltán and Bakos, Csaba and Szucs, Mate and Bonyár, Attila and Mukherjee, Deshabrato and Jankovics, Hajnalka and Vonderviszt, Ferenc and Petrik, Péter (2023) Ellipsometry monitoring of sensor processes based on gold nanoparticle bonded proteins. PROGRESS IN BIOMEDICAL OPTICS AND IMAGING (PROCEEDINGS OF SPIE). ISSN 1605-7422

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Abstract

The aim of our work is to develop a heavy metal detection sensor that can be used in the field to easily and accurately test water samples at the range of the strict concentration levels set by international standards. The sensor is based on a gold thin-film electrode on a silicon substrate and a genetically modified bacterial flagellin protein deposited onto the gold thin-film electrode. To ensure that the surface is adequately covered, and the protein is attached to the electrode, additional gold nanoparticles were deposited to the surface. Changes on the sensor surface were investigated by in-situ ellipsometry and cyclic voltammetry. The determination of the exact surface morphology, as well as the validation of the ellipsometric measurements were done by using scanning electron microscope.

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