Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR

Ali, Mohammad and Polgári, Dávid and Sepsi, Adél and Kontra, Levente and Dalmadi, Ágnes and Havelda, Zoltán and Sági, László and Kis, András (2024) Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR. PLANT METHODS, 20 (1). ISSN 1746-4811

Preview

Text Rapidandcost-effective....pdf Available under License Creative Commons Attribution. Download (2MB) | Preview

Abstract

Interspecific hybridisation is a powerful tool for increasing genetic diversity in plant breeding programmes. Hexaploid wheat (Triticum aestivum, 2n = 42) × barley (Hordeum vulgare, 2n = 14) intergeneric hybrids can contribute to the transfer of agronomically useful traits by creating chromosome addition or translocation lines as well as full hybrids. Information on the karyotype of hybrid progenies possessing various combinations of wheat and barley chromosomes is thus essential for the subsequent breeding steps. Since the standard technique of chromosome in situ hybridisation is labour-intensive and requires specific skills. a routine, cost-efficient, and technically less demanding approach is beneficial both for research and breeding.

Actions (login required)

Edit Item