Cell-specific STORM super-resolution imaging reveals nanoscale organization of cannabinoid signaling.

Dudok, Barna and Barna, László and Ledri, Marco and Szabó, Szilárd I. and Szabadits, Eszter and Woodhams, Stephen G. and Henstridge, Christopher and Kacskovics, Imre and Katona, István (2015) Cell-specific STORM super-resolution imaging reveals nanoscale organization of cannabinoid signaling. NATURE NEUROSCIENCE, 18 (1). pp. 75-86. ISSN 1097-6256

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A major challenge in neuroscience is to determine the nanoscale position and quantity of signaling molecules in a cell type- and subcellular compartment-specific manner. We developed a new approach to this problem by combining cell-specific physiological and anatomical characterization with super-resolution imaging and studied the molecular and structural parameters shaping the physiological properties of synaptic endocannabinoid signaling in the mouse hippocampus. We found that axon terminals of perisomatically projecting GABAergic interneurons possessed increased CB1 receptor number, active-zone complexity and receptor/effector ratio compared with dendritically projecting interneurons, consistent with higher efficiency of cannabinoid signaling at somatic versus dendritic synapses. Furthermore, chronic Delta9-tetrahydrocannabinol administration, which reduces cannabinoid efficacy on GABA release, evoked marked CB1 downregulation in a dose-dependent manner. Full receptor recovery required several weeks after the cessation of Delta9-tetrahydrocannabinol treatment. These findings indicate that cell type-specific nanoscale analysis of endogenous protein distribution is possible in brain circuits and identify previously unknown molecular properties controlling endocannabinoid signaling and cannabis-induced cognitive dysfunction.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Depositing User: MTMT SWORD
Date Deposited: 08 Jan 2015 15:18
Last Modified: 08 Jan 2015 15:18

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