Andrásfalvy, Bertalan and Galinanes, G. L. and Huber, D. and Barbic, M. and Macklin, J. J. and Makara, Judit (2014) Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology. NATURE METHODS, 11 (12). pp. 1237-1241. ISSN 1548-7091
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Abstract
Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RATS; *Quantum Dots; Patch-Clamp Techniques/instrumentation/*methods; Optics and Photonics/*instrumentation/methods; Neurons/cytology/*physiology; Microscopy, Fluorescence/instrumentation/*methods; MICE; Luminescent Proteins/*metabolism; Fluorescent Dyes/diagnostic use; Electrophysiology/instrumentation/*methods; Brain/cytology/*physiology; Animals |
| Subjects: | R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 24 Feb 2015 13:06 |
| Last Modified: | 30 Jun 2015 23:15 |
| URI: | http://real.mtak.hu/id/eprint/22029 |
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