Critical role of extracellular vesicles in modulating the cellular effects of cytokines

Szabó, Géza Tamás and Tarr, B. and Pálóczi, Krisztina and L. Éder, Katalin and Lajkó, Eszter and Kittel, Ágnes and Tóth, Sára and György, Bence and Szente-Pásztói, Mária and Németh, Andrea and Pállinger, Éva and Falus, András and Szabó-Taylor, Katalin and Buzás, Edit Irén (2014) Critical role of extracellular vesicles in modulating the cellular effects of cytokines. CELLULAR AND MOLECULAR LIFE SCIENCES, 71 (20). pp. 4055-67. ISSN 1420-682X


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Under physiological and pathological conditions, extracellular vesicles (EVs) are present in the extracellular compartment simultaneously with soluble mediators. We hypothesized that cytokine effects may be modulated by EVs, the recently recognized conveyors of intercellular messages. In order to test this hypothesis, human monocyte cells were incubated with CCRF acute lymphoblastic leukemia cell line-derived EVs with or without the addition of recombinant human TNF, and global gene expression changes were analyzed. EVs alone regulated the expression of numerous genes related to inflammation and signaling. In combination, the effects of EVs and TNF were additive, antagonistic, or independent. The differential effects of EVs and TNF or their simultaneous presence were also validated by Taqman assays and ELISA, and by testing different populations of purified EVs. In the case of the paramount chemokine IL-8, we were able to demonstrate a synergistic upregulation by purified EVs and TNF. Our data suggest that neglecting the modulating role of EVs on the effects of soluble mediators may skew experimental results. On the other hand, considering the combined effects of cytokines and EVs may prove therapeutically useful by targeting both compartments at the same time.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
R Medicine / orvostudomány > RZ Other systems of medicine / orvostudomány egyéb területei
Depositing User: MTMT SWORD
Date Deposited: 25 Feb 2015 03:42
Last Modified: 31 Oct 2015 00:15

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