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Efflux Pump Inhibiting Properties of Racemic Phenothiazine Derivatives and their Enantiomers on the Bacterial AcrAB-TolC System

Spengler, Gabriella and Takács, Daniella and Horváth, Ádám and Szabó, Ágnes Míra and Riedl, Zsuzsanna and Hajós, György and Molnár, József and Burián, Katalin (2014) Efflux Pump Inhibiting Properties of Racemic Phenothiazine Derivatives and their Enantiomers on the Bacterial AcrAB-TolC System. In Vivo, 28 (6). pp. 1071-1075. ISSN 0258-851X

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Abstract

Background/Aim: Bacterial resistance to antibiotics has become a serious problem in antibacterial chemotherapy and resistance of bacteria to chemicallyunrelated anti-microbial agents can be associated with the over-expression of efflux pumps. The simultaneous therapy with efflux pump inhibitors (EPIs) could be a solution to improve the effectiveness of antibiotics. The response of an organism to an EPI often depends on how that molecule fits a particular site of a protein. Because enantiomers of a given compound rotate plane-polarized light in a solution by the same angle but in opposite directions, the rational drug design should take the chirality into account if there is a difference between the racemic compound and its enantiomers. Materials and Methods: The main goal of the present study was to elucidate the role of chirality of Nhydroxyalkyl- 2-aminophenothiazines as effective EPIs by an automated method that uses the general efflux pump substrate ethidium bromide (EB) for the assessment of AcrAB-TolC system of wild-type Escherichia coli K-12 AG100. It has been shown that the most active EPIs among the N-hydroxyalkyl-2-aminophenothiazines were the compounds rac-3i, (+)-3i, and (–)-3i by modulating the AcrAB-TolC efflux pump. Conclusion: Comparison of effects of enantiomeric pairs revealed that their activities were similar to that of racemic derivatives. Moreover, there was no significant difference between the racemic compounds and their enantiomers related to their antibacterial and efflux pump inhibiting effects.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD04 Organic chemistry / szerves kémia
Depositing User: Dr. xAranka xPilbáth
Date Deposited: 11 Mar 2015 13:40
Last Modified: 11 Mar 2015 13:40
URI: http://real.mtak.hu/id/eprint/22420

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