Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. paratuberculosis strains from various sources.

Rónai, Zsuzsanna and Csivincsik, Ágnes and Gyuranecz, Miklós and Kreizinger, Z. and Dán, Ádám (2014) Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. paratuberculosis strains from various sources. JOURNAL OF APPLIED MICROBIOLOGY, 118. pp. 275-283. ISSN 1364-5072

[img] Text
Restricted to Repository staff only

Download (289kB) | Request a copy


AIMS: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease. Genotypic discrimination of MAP isolates is pivotal to epidemiological studies requisite for revealing infection sources and disease transmission. This study was undertaken to determine the genetic diversity of MAP strains from diverse sources. METHODS AND RESULTS: 569 MAP isolates were collected during an eight-year period from 9 animal species, originating from 7 European countries, including the whole geographic region of Hungary. Isolates were classified into cattle- and sheep-types and 515 strains were included in Mycobacterial Interspersed Repetitive Units - Variable-Number Tandem Repeat analysis. CONCLUSIONS: The same genotype was found in different host species co-habiting on the same property, demonstrating interspecies transmission. Detecting identical patterns in numerous related animals underlines the importance of vertical transmission. The revealed 15 genotypes expose relatively low strain diversity, and indicate the need of an improved typing system that provides higher resolution in the case of this subspecies. SIGNIFICANCE AND IMPACT OF STUDY: Our results demonstrate the circulation and transmission of different MAP strain types among individuals, herds and even wildlife reservoirs in Hungary and other European countries; and correlation between production type or breed and MAP genotype is hypothesised. This article is protected by copyright. All rights reserved.

Item Type: Article
Additional Information:
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: MTMT SWORD
Date Deposited: 23 Jul 2015 08:52
Last Modified: 23 Jul 2015 08:52

Actions (login required)

Edit Item Edit Item