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Temperature-dependent transformation of biogas-producing microbial communities points to the increased importance of hydrogenotrophic methanogenesis under thermophilic operation

Pap, Bernadett and Györkei, Ádám and Boboescu, IZ and Nagy, Ildikó Katalin and Bíró, Tibor and Kondorosi, Éva and Maróti, Gergely (2015) Temperature-dependent transformation of biogas-producing microbial communities points to the increased importance of hydrogenotrophic methanogenesis under thermophilic operation. BIORESOURCE TECHNOLOGY, 177. pp. 375-380. ISSN 0960-8524

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Abstract

Stability of biogas production is highly dependent on the microbial community composition of the bioreactors. This composition is basically determined by the nature of biomass substrate and the physical-chemical parameters of the anaerobic digestion. Operational temperature is a major factor in the determination of the anaerobic degradation process. Next-generation sequencing (NGS)-based metagenomic approach was used to monitor the organization and operation of the microbial community throughout an experiment where mesophilic reactors (37. °C) were gradually switched to thermophilic (55. °C) operation. Temperature adaptation resulted in a clearly thermophilic community having a generally decreased complexity compared to the mesophilic system. A temporary destabilization of the system was observed, indicating a lag phase in the community development in response to temperature stress. Increased role of hydrogenotrophic methanogens under thermophilic conditions was shown, as well as considerably elevated levels of Fe-hydrogenases and hydrogen producer bacteria were observed in the thermophilic system.

Item Type: Article
Uncontrolled Keywords: Thermotogae; thermophilic bacterium; Thermoanaerobacteriales; Temperature stress; temperature acclimatization; TEMPERATURE; Syntrophomonas wolfei; Syntrophomonas; PROTEOBACTERIA; Prevotella; physical parameters; Parabacteroides; OXIDATION; nonhuman; microbial metabolism; Methanothermobacter; Methanospirillum; Methanosarcina; Methanosaeta; methanogenic archaeon; Methanogen; Methanoculleus; Firmicutes; ecosystem; Desulfotomaculum; DEGRADATION; DECOMPOSITION; controlled study; concentration (parameters); COMMUNITY STRUCTURE; Clostridium thermocellum; Clostridium sporogenes; Clostridium cellulolyticum; chemical parameters; BIOMASS; biofuel production; Bacteroidetes; bacteroides; ARTICLE; TRANSFORMATION; thermophily; temperature effect; Physicochemical property; MICROBIAL COMMUNITY; methanogenesis; gas production; experimental design; Community composition; BIOREACTOR; anoxic conditions; IRON HYDROGENASE; HYDROGEN; Physical-chemical parameters; Next-generation sequencing; Microbial community composition; MICROBIAL COMMUNITIES; Hydrogenotrophic methanogens; Hydrogenases; MICROORGANISMS; Biogas; Thermophilic microbial community; Metagenomics; Hydrogenotrophic methanogenesis; hydrogenase; anaerobic digestion
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 24 Sep 2015 14:40
Last Modified: 24 Sep 2015 14:40
URI: http://real.mtak.hu/id/eprint/27744

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