Pap, Bernadett and Györkei, Ádám and Boboescu, IZ and Nagy, Ildikó Katalin and Bíró, Tibor and Kondorosi, Éva and Maróti, Gergely (2015) Temperature-dependent transformation of biogas-producing microbial communities points to the increased importance of hydrogenotrophic methanogenesis under thermophilic operation. BIORESOURCE TECHNOLOGY, 177. pp. 375-380. ISSN 0960-8524
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Abstract
Stability of biogas production is highly dependent on the microbial community composition of the bioreactors. This composition is basically determined by the nature of biomass substrate and the physical-chemical parameters of the anaerobic digestion. Operational temperature is a major factor in the determination of the anaerobic degradation process. Next-generation sequencing (NGS)-based metagenomic approach was used to monitor the organization and operation of the microbial community throughout an experiment where mesophilic reactors (37. °C) were gradually switched to thermophilic (55. °C) operation. Temperature adaptation resulted in a clearly thermophilic community having a generally decreased complexity compared to the mesophilic system. A temporary destabilization of the system was observed, indicating a lag phase in the community development in response to temperature stress. Increased role of hydrogenotrophic methanogens under thermophilic conditions was shown, as well as considerably elevated levels of Fe-hydrogenases and hydrogen producer bacteria were observed in the thermophilic system.
Item Type: | Article |
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Uncontrolled Keywords: | Thermotogae; thermophilic bacterium; Thermoanaerobacteriales; Temperature stress; temperature acclimatization; TEMPERATURE; Syntrophomonas wolfei; Syntrophomonas; PROTEOBACTERIA; Prevotella; physical parameters; Parabacteroides; OXIDATION; nonhuman; microbial metabolism; Methanothermobacter; Methanospirillum; Methanosarcina; Methanosaeta; methanogenic archaeon; Methanogen; Methanoculleus; Firmicutes; ecosystem; Desulfotomaculum; DEGRADATION; DECOMPOSITION; controlled study; concentration (parameters); COMMUNITY STRUCTURE; Clostridium thermocellum; Clostridium sporogenes; Clostridium cellulolyticum; chemical parameters; BIOMASS; biofuel production; Bacteroidetes; bacteroides; ARTICLE; TRANSFORMATION; thermophily; temperature effect; Physicochemical property; MICROBIAL COMMUNITY; methanogenesis; gas production; experimental design; Community composition; BIOREACTOR; anoxic conditions; IRON HYDROGENASE; HYDROGEN; Physical-chemical parameters; Next-generation sequencing; Microbial community composition; MICROBIAL COMMUNITIES; Hydrogenotrophic methanogens; Hydrogenases; MICROORGANISMS; Biogas; Thermophilic microbial community; Metagenomics; Hydrogenotrophic methanogenesis; hydrogenase; anaerobic digestion |
Subjects: | Q Science / természettudomány > Q1 Science (General) / természettudomány általában |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 24 Sep 2015 14:40 |
Last Modified: | 24 Sep 2015 14:40 |
URI: | http://real.mtak.hu/id/eprint/27744 |
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