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A COMPARISON OF 2-POINT, 3-POINT AND DELETION MAPPING IN THE C-CISTRON OF RHIZOBIOPHAGE-16-3, WITH AN EXPLANATION FOR THE RECOMBINATION PATTERN

Orosz, László and Rostas, Katalin and Hotchkiss, Rollin D. (1980) A COMPARISON OF 2-POINT, 3-POINT AND DELETION MAPPING IN THE C-CISTRON OF RHIZOBIOPHAGE-16-3, WITH AN EXPLANATION FOR THE RECOMBINATION PATTERN. Genetics, 94 (2). pp. 249-263. ISSN 0016-6731 (print), 1943-2631 (online)

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Abstract

A general tendency for additivity prevailed in recombination frequencies for two-point fine-structure mapping of 14 mutants in the C cistron of Rhizobium meliloti phage 16-3, with little evidence of any marker effect. Intracistronic three-point mapping indicated that double crossovers are rare. Deletion mapping indicated that the two- and three-point mapping data gave the correct order of the mutations. A high frequency (5 to 8%) of c/c+ heterozygotic phage progenies was observed in standard crosses. This pattern implies formation of a relatively long region of heterozygosity. Together with the results of the three-point tests, it suggests certain properties of the branch migration and resolution steps envisioned in current mechanisms of recombination.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
Depositing User: Erika Bilicsi
Date Deposited: 13 Nov 2012 07:46
Last Modified: 13 Nov 2012 07:46
URI: http://real.mtak.hu/id/eprint/3343

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