Molecular screening for Anaplasmataceae in ticks and tsetse flies from Ethiopia

Hornok, Sándor and Abichu, Getachew and Takács, Nóra and Gyuranecz, Miklós and Farkas, Róbert and Fernández De Mera, Isabel G. and De La Fuente, José (2016) Molecular screening for Anaplasmataceae in ticks and tsetse flies from Ethiopia. Acta Veterinaria Hungarica, 64 (1). pp. 65-70. ISSN 0236-6290


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Hard ticks and tsetse flies are regarded as the most important vectors of disease agents in Sub-Saharan Africa. With the aim of screening these blood-sucking arthropods for vector-borne pathogens belonging to the family Anaplasmataceae in South-Western Ethiopia, four species of tsetse flies (collected by traps) and seven species of ixodid ticks (removed from cattle) were molecularly analysed. DNA was extracted from 296 individual ticks and from 162 individuals or pools of tsetse flies. Besides known vector–pathogen associations, in Amblyomma cohaerens ticks sequences of Anaplasma marginale and A. phagocytophilum were detected, the latter for the first time in any ticks from cattle in Africa. In addition, part of the gltA gene of Ehrlichia ruminantium was successfully amplified from tsetse flies (Glossina pallidipes). First-time identification of sequences of the above pathogens in certain tick or tsetse fly species may serve as the basis of further epidemiological and transmission studies.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: Erika Bilicsi
Date Deposited: 02 Jun 2016 04:29
Last Modified: 31 Mar 2017 23:15

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