Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine max L.) through inducing antioxidant system

Guler, Neslihan Saruhan and Pehlivan, Necla (2016) Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine max L.) through inducing antioxidant system. Acta Biologica Hungarica, 67 (2). pp. 169-183. ISSN 0236-5383


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Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) functions as a signal molecule in plants under abiotic and biotic stress. In this study, the role of exogenous H<sub>2</sub>O<sub>2</sub> in improving drought tolerance in two soybean cultivars (Glycine max L. Merrill) differing in their tolerance to drought was evaluated. Plants were grown in plastic pots with normal irrigation in a phytotron. Four weeks after radicle emergence, either 1 mM H<sub>2</sub>O<sub>2</sub> or distilled water was sprayed as foliar onto the leaves of each plant, after drought stress was applied. Leaf samples were harvested on the 4<sup>th</sup> and 7<sup>th</sup> days of the drought. Antioxidant-related enzyme activity, such as the superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) content was measured during the drought period. Drought stress decreased leaf water potential, relative water content and photosynthetic pigment content but enhanced lipid peroxidation and endogenous H<sub>2</sub>O<sub>2</sub> concentration. By contrast, exogenous low dose H<sub>2</sub>O<sub>2</sub> improved water status, pigment content and lipid peroxidation under drought stress. Endogenous H<sub>2</sub>O<sub>2</sub> concentration was reduced by exogenous H<sub>2</sub>O<sub>2</sub> as compared to drought treatment alone. H<sub>2</sub>O<sub>2</sub> pre-treatment induced all the antioxidant enzyme activities, to a greater extent than the control leaves, during drought. H<sub>2</sub>O<sub>2</sub> pretreatment further enhanced the activities of antioxidant enzymes in the tolerant cultivar compared to the sensitive cultivar. Results suggested that low dose H<sub>2</sub>O<sub>2</sub> pre-treatment alleviated water loss and H<sub>2</sub>O<sub>2</sub> content and increased drought stress tolerance by inducing the antioxidant system.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Depositing User: Ágnes Sallai
Date Deposited: 08 Jun 2016 11:17
Last Modified: 30 Jun 2017 23:15

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