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Structural plasticity of the Salmonella FliS flagellar export chaperone

Sajó, Ráchel and Tőke, Orsolya and Hajdú, István and Jankovics, Hajnalka and Micsonai, András and Dobó, József and Kardos, József (2016) Structural plasticity of the Salmonella FliS flagellar export chaperone. FEBS LETTERS, 590 (8). pp. 1103-1113. ISSN 0014-5793

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Abstract

The Salmonella FliS flagellar export chaperone is a highly α-helical protein. Proteolytic experiments suggest that FliS has a compact core. However, the calorimetric melting profile of FliS does not show any melting transition in the 25-110 °C temperature range. CD measurements reveal that FliS is losing its helical structure over a broad temperature range upon heating. These observations indicate that FliS unfolds in a non-cooperative way and its native state shows features reminiscent of the molten globule state of proteins possessing substantial structural plasticity. As FliS has several binding partners within the cell, conformational adaptability seems to be an essential requirement to fulfill its multiple roles. This article is protected by copyright. All rights reserved.

Item Type: Article
Uncontrolled Keywords: atypical molten globule; non-cooperative unfolding; flagellar export system; FliS; Flagellar export chaperone
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD04 Organic chemistry / szerves kémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 09 Jun 2016 14:49
Last Modified: 01 Apr 2017 23:15
URI: http://real.mtak.hu/id/eprint/36199

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