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Characterization of Human Pituitary Adenomas in Cell Cultures by Light And Electron Microscopic Morphology And Immunolabeling

Fazekas, Ilona and Hegedűs, Balázs and Bácsy, Ernő and Kerekes, Edit and Slowik, Felicia and Bálint, Katalin and Pásztor, Emil (2005) Characterization of Human Pituitary Adenomas in Cell Cultures by Light And Electron Microscopic Morphology And Immunolabeling. Folia Histochemica et Cytobiologica, 43 (2). pp. 81-90. ISSN 0239-8508 (print), 1897-5631 (online)

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Abstract

The morphology and hormone production of pituitary adenoma cell cultures were compared in order to highlight their characteristic in vitro features. Cell suspensions were prepared from 494 surgical specimens. The 319 viable monolayer cultures were analyzed in detail by light microscopy and immunocytochemistry within two weeks of cultivation. Some cultures were further characterized by scanning, transmission and immunogold electron microscopy. The viability and detailed in vitro morphology of adenoma cells were found to be characteristic for the various types of pituitary tumors. The sparsely granulated growth hormone, the corticotroph and the acidophil stem cell adenomas provided the highest ratio of viable cultures. Occasionally, prolonged maintenance of cells resulted in long-term cultures. Furthermore, a variety of particular distributions of different hormone-containing granules were found in several cases. Both light microscopic and ultrastructural analyses proved that the primary cultures of adenoma cells retain their physiological features during in vitro cultivations. Our in vitro findings correlated with the routine histopathological examination. These results prove that monolayer cultures of pituitary adenoma cells can contribute to the correct diagnosis and are valid model systems for various oncological and neuroendocrinological studies.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Depositing User: Erika Bilicsi
Date Deposited: 11 Jan 2013 11:35
Last Modified: 11 Jan 2013 11:35
URI: http://real.mtak.hu/id/eprint/3876

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