Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria

Süle, Judit and Kőrösi, T. and Hucker, A. and Varga, László (2014) Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria. BRAZILIAN JOURNAL OF MICROBIOLOGY, 45 (3). pp. 1023-1030. ISSN 1517-8382


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The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides- mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumer- ation of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus , however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or ex- ceeding those of Lb. acidophilus . Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selec- tive enumeration of Lactobacillus spp.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: MTMT SWORD
Date Deposited: 11 Sep 2016 07:45
Last Modified: 11 Sep 2016 07:45

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