REAL

TP53 mutation hits energy metabolism and increases glycolysis in breast cancer.

Harami-Papp, Hajnalka and Pongor, Lőrinc S. and Munkácsy, Gyöngyi and Horváth, Gergő and M. Nagy, Ádám and Hauser, Péter and Szabó, András and Győrffy, Balázs (2016) TP53 mutation hits energy metabolism and increases glycolysis in breast cancer. Oncotarget. pp. 1-13. ISSN 1949-2553

[img]
Preview
Text
11594_181594_2_PB_1_u.pdf

Download (3MB) | Preview

Abstract

Promising new hallmarks of cancer is alteration of energy metabolism that involves molecular mechanisms shifting cancer cells to aerobe glycolysis. Our goal was to evaluate the correlation between mutation in the commonly mutated tumor suppressor gene TP53 and metabolism. We established a database comprising mutation and RNA-seq expression data of the TCGA repository and performed receiver operating characteristics (ROC) analysis to compare expression of each gene between TP53 mutated and wild type samples. All together 762 breast cancer samples were evaluated of which 215 had TP53 mutation. Top up-regulated metabolic genes include glycolytic enzymes (e.g. HK3, GPI, GAPDH, PGK1, ENO1), glycolysis regulator (PDK1) and pentose phosphate pathway enzymes (PGD, TKT, RPIA). Gluconeogenesis enzymes (G6PC3, FBP1) were down-regulated. Oxygen consumption and extracellular acidification rates were measured in TP53 wild type and mutant breast cell lines with a microfluorimetric analyzer. Applying metabolic inhibitors in the presence and absence of D-glucose and L-glutamine in cell culture experiments resulted in higher glycolytic and mitochondrial activity in TP53 mutant breast cancer cell lines. In summary, TP53 mutation influences energy metabolism at multiple levels. Our results provide evidence for the synergistic activation of multiple hallmarks linking to these the mutation status of a key driver gene.

Item Type: Article
Subjects: R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0254 Neoplasms. Tumors. Oncology (including Cancer) / daganatok, tumorok, onkológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 21 Sep 2016 13:56
Last Modified: 21 Sep 2016 13:56
URI: http://real.mtak.hu/id/eprint/39749

Actions (login required)

Edit Item Edit Item